module Bio::Tools::Run::Alignment::Sim4 !
Bonjour,
J'essaie d'utiliser le module Sim4
Bio::Tools::Run::Alignment::Sim4 - Wrapper for Sim4 program that allows for alignment of cdna to genomic sequences
mais j'obtiens l'erreur :
Citation:
Use of uninitialized value in concatenation (.) or string at C:/Perl/site/lib/Bio/Tools/Run/Alignment/Sim4.pm line 282.
Use of uninitialized value in concatenation (.) or string at C:/Perl/site/lib/Bio/Tools/Run/Alignment/Sim4.pm line 290.
et un des fichier s'ouvre
Voici mon code :
Code:
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| #!/usr/bin/perl
use strict;
use warnings;
use Bio::Tools::Run::Alignment::Sim4;
my $cdna_seq = "P:/Theorie/Driss/PCR_quintuplex/sequences/p16.fa";
my $genomic_seq = "P:/Theorie/Driss/PCR_quintuplex/ref_DNA_sequences/p16.fa";
my @params = (W=>15, K=>17, D=>10, N=>10,cdna_seq=>$cdna_seq, genomic_seq=>$genomic_seq);
my $sim4 = Bio::Tools::Run::Alignment::Sim4->new(@params);
my @exon_sets = $sim4->align; |
Merci beaucoup
