Problème avec le module Bio-PrimerDesigner

Bonjour,


J'aimerais utiliser le module Bio-PrimerDesigner.

http://search.cpan.org/~smckay/Bio-P...merDesigner.pm

Citation:

---

DESCRIPTION

Bio-PrimerDesigner provides a low-level interface to the primer3 and epcr binary executables and supplies methods to return the results. Because primer3 and e-PCR are only available for Unix-like operating systems, Bio-PrimerDesigner offers the ability to accessing the primer3 binary via a remote server. Local installations of primer3 or e-PCR on Unix hosts are also supported.

---

Néanmoins, en allant sur le site de Primer3, j'ai vu qu'il était disponible pour Windows XP. Je l'ai donc téléchargé et l'exécutable se trouve dans C:\Program Files\primer3\bin\primer3_core.exe

J'ai ensuite installé le module Bio-PrimerDesigner qui était disponible via l'interface tk de ppm ... n'est-ce pas la preuve qu'il est sensé fonctionner sous Windows?

Lorsque j'exécute le script suivant

Code:

---

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

#usr/perl/bin/   use warnings; use strict;   use Bio::PrimerDesigner;       my $pd = Bio::PrimerDesigner->new;   # # Define the DNA sequence, etc. # my $dna = "CGTGCTTCGC"; my $seqID = "sequence 1";   # # Define design parameters (input aliases) # native primer3 syntax # my %params = ( num => 2, # PRIMER_NUM_RETURN id => $seqID, # PRIMER_SEQUENCE_ID seq => $dna, # SEQUENCE sizerange => '500-600' # PRIMER_PRODUCT_SIZE );       # # Design primers # my $results = $pd->design( %params ) or die $pd->error;     # Can't execute local binary '/usr/local/bin/primer3' at test.pl line 33.

---

J'obtiens l'erreur

Citation:

---

Can't execute local binary '/usr/local/bin/primer3' at test.pl line 33.

---

Il y a donc un problème à la dernière ligne du script quand Perl essaie de trouver l'exécutable ... est-ce un problème de nom? Mon exécutable se nomme primer3_core.exe et Perl semble attendre un primer3.exe.
Est-ce un problème de localisation? Tous les sous-répertoires 'bin' situés dans 'Program Files' sont-ils répertoriés dans /usr/local/bin/?
Comment cela fonctionne-t'il?


Merci,

Citation:

---

How to install this software
============================

1. Unzip the '.zip' file downloaded from SourceForge.net
2. You will create a primer3-1.1.x folder in the location where the file was unzipped
3. You may copy the files from the 'bin' directory of the primer3-1.1.x folder to a location of your choice. The exact same files are located within the 'src' folder so that the tests may be run (windows does not allow relative paths in shortcuts).

Running the tests
=================
You must install a perl distribution to run the windows tests.

We *strongly* recommend you install ActiveState perl (http://www.activestate.com/products/activeperl/)
as this was used to test our primer3 builds, and it is known to work.

***The perl test script for windows has a different name, at the moment, than that for the
unix/linux versions. See below.***

1. Click on 'Start > Run...'
2. Type 'cmd' into the space provided
3. Hit enter (or select 'OK')
4. Navigate to the location of the tests:

A. if you put it in C:/Documents and Settings/YourName/primer3-1.1.x/test/,
you would type 'cd c:/Documents and Settings/YourName/primer3-1.1.x/test/'

B. you can also type 'cd ' (don't forget the space after cd)
and drag the primer3-1.1.x folder onto the command-line window from
windows explorer, this will fill in the location for you

5. On the command line, run 'perl p3test.pl --windows' in this directory
6. You should see [OK] for all of the tests.

---

C'est apparemment prévu qu'on déplace les exécutables, je suis entrain d'exécuter les tests, jusqu'à présent tout semble correct.