Microscope image analysis

Dear all,

i'm trying to analyze microscope images with Matlab.

-> What i would like:
Starting from an image, like img_di_partenza (attached, not the original resolution), i would like to filter it and highlight only the fibers.
Pièce jointe 605711
-> What i managed to achieve
- First thing, i do is bring the grayscale image with rgb2gray.
- Increase the contrast with imadjust
- Finally I have set ‘manual threshold’.
I get more or less something like img_pos_elabora image.
N.B. the two images uploaded are not the same, they are an example.
Pièce jointe 605712
-> Problems
- The result has a high 'signal to noise ratio'.
- I have many images and therefore I would like to automate everything (I would like to skip the step of the manual threshold).
Can someone help me?

You should try the PCA with imagej to retrieve all vertices

Citation:

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Envoyé par mach1974

You should try the PCA with imagej to retrieve all vertices

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I tried using same imageJ plugins, but i don't know what is PCA.
What is PCA ?

TY

Imagej can provide you the source .
It consists in
firstable : convert the image in grey level
Then the system will consider the image like a matrix and calculate the inverse of image
The asset goes back to highlight the vertices