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| #!/usr/bin/python3
#-*-coding:Utf-8-*-
import csv
######
#Calcul de fréquences
def frequence(nb,total):
"""Calculate a frequency. If total=0, return 0
"""
try:
return nb/total
except ZeroDivisionError:
return 0
######
#Tableau séquence de référence/coverage
def tableau_init(file_ref, file_cov):
#Lecture de la séquence de référence
seq = []
_open = open(file_ref, "r")
read = _open.readlines()
for line in read:
if line[0] == ">":
ID = line[1:]
ID = ID.split()
ID = ID[0].split('|')
ID = ID[len(ID)-1].lower() #Nom de la seq de référence utilisée
else :
for i in line :
i = i.upper()
i = i.strip()
if i != "":
seq.append(i)
else :
pass
#Lecture du coverage
coverage = []
_open = open(file_cov, "r")
read = csv.reader(_open)
for line in read:
line = " ".join(line)
line = line.replace(";","").replace('"'," ")
line = line.split()
if "Value" in line:
pass
else:
line = [int(i) for i in line]
coverage.append(line[1])
#Création du tableau
tableau = []
for i in list(range(len(seq))): #Position et seq. ref et consensus + reads
(pos_cons, seq_cons, A, T, G, C, GAP) = ("-", seq[i], 0, 0, 0, 0, 0)
line = [i+1, seq[i], coverage[i]]
if seq[i] == "A":
A = coverage[i]
elif seq[i] == "T":
T = coverage[i]
elif seq[i] == "G":
G = coverage[i]
elif seq[i] == "C":
C = coverage[i]
line = [i+1, seq[i], coverage[i], pos_cons, seq_cons, A, T, G, C, GAP]
tableau.append(line)
return tableau
#Tableau des conflits
def tableau_conf(file_conf):
tableau = []
_open = open(file_conf, "r")
read = csv.reader(_open, delimiter=';')
for line in read: #lecture ligne par ligne
if "^" in line[0]: #ignore les insertion
pass
else:
line = " ".join(line)
line = line.replace(".", "").replace("(","").replace(")","").replace(",","")
line = line.split()
(pos_ref, pos_cons, seq_cons) = (line[0], line[1], line[2])
i = 6 #comptage des reads
taille = len(line)-2
(A, T, G, C, GAP) = (0,0,0,0,0)
while (i < taille):
if line[i] == "A" or line[i] == "AA":
i=i+1
A = line [i]
elif line[i] == "T" or line[i] == "TT":
i=i+1
T = line [i]
elif line[i] == "G" or line[i] == "GG":
i=i+1
G = line [i]
elif line[i] == "C" or line[i] == "CC":
i=i+1
C = line [i]
elif line[i] == "-" or line[i] == "--":
i=i+1
GAP = line [i]
i = i+1
line_seq = [line[0], line[1], line[2]]
line_data = [A, T, G, C, GAP]
line_data = [int(i) for i in line_data]
line = line_seq + line_data
tableau.append(line)
return tableau
#Tableau séquence de référence/coverage/conflits
def tableau_all(file_seq, file_cov, file_conf):
tab_init = tableau_init(file_seq, file_cov)
tab_conf = tableau_conf(file_conf)
for i in tab_conf[1:]:
numero = int(i[0])-1
tab_init[numero][3:] = i[1:]
return tab_init
#Fréquences
def tableau_freq(file_seq, file_cov, file_conf):
tableau = tableau_all(file_seq, file_cov, file_conf)
for i in tableau:
# (nbA, nbT, nbG, nbC, nbGAP, nbcov) = (i[5], i[6], i[7], i[8], i[9], i[2])
total = i[2] - i[9]
freqA = frequence(i[5], total)
freqA = str(freqA).replace(".", ",")
i.append(freqA)
freqT = frequence(i[6], total)
freqT = str(freqT).replace(".", ",")
i.append(freqT)
freqG = frequence(i[7], total)
freqG = str(freqG).replace(".", ",")
i.append(freqG)
freqC = frequence(i[8], total)
freqC = str(freqC).replace(".", ",")
i.append(freqC)
return tableau
#Création du tableau csv
def tab_csv(file_seq, file_cov, file_conf, filetest):
if "csv" in filetest:
pass
else:
filetest = filetest + ".csv"
file_out = open(filetest, "w")
writer = csv.writer(file_out, delimiter = ";")
tableau = tableau_freq(file_seq, file_cov, file_conf)
writer.writerow(["Position","Seq_ref","Coverage","Pos_consensus","Seq_consensus","nbA","nbT","nbG","nbC","nbGAP", "freqA", "freqT", "freqG", "freqC"])
for i in list(range(len(tableau))):
writer.writerow(tableau[i]) |
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